O-34: Cytoplasmic Transplantation in Oocytes Obtained from Ovarian Tissue Xenotransplantation Lead to Reduced Apoptosis and Higher Fertilization Rate

O-17: Human Ovarian Tissue Xenotransplantation Application in Drug Discover

Background: Reproductive toxicity studies (RTS) represent an important part of pre-clinical safety evaluation of any drug development process for human consumption. It is mandatory requirement that RTS must be completed before any drug can be administered to women of childbearing age. Therefore, at first segment of toxicity check the toxic effect of the drug on fertility must be tested on non-c...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

O-4: Morphological Analyses and Apoptosis Genes Expression Evaluation in Vitrified Human Ovarian Tissue after Warming, Long Term Culturing and Xenotransplantation

Background In vitro culturing and retransplantion of vitrified- warmed ovarian tissue are two ways to restore fertility after radiation or chemotherapy.This study aimed to evaluate the incidence of apoptosis in vitrified human ovarian tissue after warming, long term culturing and xenotransplantation by morphological analyses and apoptosis genes expression evaluation. MaterialsAndMethods We obta...

P-78: Role of Allopurinol, as An Antioxidant Factor, in Increasing The Number of Received Oocytes and Embryos, and Reduce Apoptosis after Heterotopic Transplantation Mouse Ovarian Tissue

Background: Ischemia and reperfusion after transplantation is the main problem which decreases follicular density in the grafted ovarian. Many sources of free radicals such as xanthine oxidase were generated during ischemia. In this study, we used allopurinol as xanthine oxidase inhibitor to reduce ischemia-reperfusion injury, to increase received oocytes and embryos, and to decrease Apoptosis ...

Assessment of the integrity of human oocytes retrieved from cryopreserved ovarian tissue after xenotransplantation.

BACKGROUND Previous studies showed that immature oocytes stored in ovarian tissue could develop to the mature stage after transplantation. However, the quality and competency of the oocytes developed in xenografted ovarian tissue have never been investigated. As a pilot study to investigate this uncharted issue, we evaluated microtubule organization and chromatin configuration of human oocytes ...